Title
Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A
Publication Title
Carbohydrate Research
Abstract
The enzyme endo-polygalacturonase A, or PGA, is produced by the fungus, Aspergillus niger, and appears to play a critical role during invasion of plant cell walls. The enzyme has been homologously overexpressed in order to provide sufficient quantities of purified enzyme for structural and biological studies. We have characterized this enzyme in terms of its post-translational modifications (PTMs) and found it to be both N- and O-glycosylated. Additionally, we have characterized the glycosyl moieties using MALDI-TOF and LC–ESI mass spectrometry. The characterization of all PTMs on PGA, along with molecular modeling, allows us to reveal potential roles played by the glycans in modulating the interaction of the enzyme with other macromolecules.
Publication Date
10-16-2006
Page Range
2370-2378
Document Type
Published Article
DOI
https://doi.org/10.1016/j.carres.2006.06.006
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